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Dis-infection and sterilization

Principle of Dis-infection and Sterilization

Dis-infection and sterilization is important for Prevention and Infection in Hospital. Re-usable Medical Device used for treatment must be obtained effectively Dis-infection and Sterilization.
According to the standard for patient safety.

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Spaulding Classification

Classification

  Dis - infection Level

Effectiveness

Example

Critical  Item; 
Items  that in contact with sterilization tissue or  vascular system                        

• Sterilization  level

•  Kills  all micro - organism life

• Surgical  instrument
• Implant
• Biopsy

Se-mi  critical;
Items  that in contact with Mucous membrane or non - intact skin

• Sterilization
• High level  dis-infection

• Kills  all micro - organism but not  necessarily all bacterial spores 

• Flexible  endoscope
• Respiratory   equipment
• Anesthesia  equipment

Non-Critical ;
Items  that in contact with intact skin

• Intermediate - level   dis - infection
• Low level  dis-infection

• Kills  virus ,bacterial, Fungi  

• EKG
• BP  cuff

Classification Medical device

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Dis-infection

Dis-infection is Kill all micro-organism on medical device surface but can not kill prion or mycobacteria by heat, chemical and Dis-infectant

There are 3 level of Dis-infection

  • 1. Low-level dis-infection
    • Destruction can destroy bacterial, some virus and fungi
    • but cannot destroy durable bacteria such as tuberculosis
  • 2. Inter-mediated dis-infection
    • Do not destroy bacteria spores
  • 3. High-level dis-infection
    • Can destroy bacterial. All type of virus, fungi and mycobacteria

Temperature and duration Dis-infection

High level Dis-infectant

Temperature & Duration
(High level dis-infection)

Temperature & Duration
(Sterilization)

7.5% Hydrogen peroxide

Temperature 20 c Duration 30 min Temperature 20 c Duration 6 hours

0.2% Peracetic acid

-

Temperature 50-56 c Duration 12 min

>= 2% Glutaraldehyde

Temperature 20-25 c Duration 20-90 min

Temperature 20-25 c Duration 10 hours
0.55% Otho phthalaldehyde (OPA) Temperature 20-25 c Duration 5 min

-

7.35% Hydrogen peroxide / 0.23% peracetic acid

Temperature 20 c Duration 15 min

Temperature 20 c Duration 3 hours

Micro-organism resistant

Resistance

Micro-organism

Dis-infection level

The most Prion (Creutzfeldt – jakob disease) Prion  reprocessing
Bacterial  spores (C.difficile) Sterilization

Mycobacterial

High level dis-infection
Small , non-envelope viruses (HP, polio, EV-D68)

Intermediate-level dis-infection

Fungal spores (Aspergillus , Canada)

Intermediate-level dis-infection
Gram negative bacilli (Acenetobacter)

Intermediate-level dis-infection

Vegetative fungi and algae

Low-level dis-infection
Large , non-envelope viruses

Low-level dis-infection

Gram positive bacteria (MRSA ,VRE)

Low-level dis-infection
Least Envelope viruses (Ebola .MERS- CoV)

Low-level dis-infection

What is Sterilization ???

  • Sterilization is the process used to render a medical device free from all forms of viable micro-organisms (Bacteria, Spore, Fungi and Viruses)
  • Sterilization is required not all micro-organisms have been completely destroyed during the contamination process
  • Within the Sterile processing department, the sterilization are is where sterilizer (steam, low temperature), are located including the space for loading, unloading, and cooling. This can be accommodated by separated three zone system.

Sterile processing Zoning

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Variables that Sterilization

  • The dryness of medical device to be processed
  • The temperature and humidity of the process area
  • Medical device were properly prepared and loaded in to sterilizer
  • The sterilant is properly in process
  • Sterilizer Maintenance protocoz
  • The correct sterilization method and cycle were used
  • Knowledge and skill of operation staff

Sterilization Process

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Sterilization Method

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Monitoring of sterilization

Sterilizer Mechanical
Monitoring
Chemical monitoring Biological test
External Internal Bowie Dick test
Steam
Sterrad -
Hot Air Oven - - -

Sterilization of Quality Assurance

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Living - Healthy - Daily

Living is an art,
Being Healthy is a journey,
Make the artistic journey Daily

 

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Contact

66 2 826 9986

bwc.dental@bdmswellness.com

Address

2/4 Wireless Road, Lumpini, Pathumwan,
Bangkok 10330

Opening hours

09.00-17.00 MON - FRI 

09.00-12.00 SAT

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